prism software package version 10  (GraphPad Software Inc)

Journal: BMC Complementary Medicine and Therapies

Article Title: Bioactivity-guided fractionation of Withania somnifera (L.) Dunal roots extract: evaluation of the anti-progressive potential on human Kelly neuroblastoma cell line

doi: 10.1186/s12906-025-05018-2

Figure Lengend Snippet: Percentage viability of Kelly cells treated with the vehicle. Cells were incubated in medium alone (control) or medium containing 0.5% DMSO (vehicle) for 96 h in 96-well plates at 37 °C. Following incubation, cell viability was assessed using the MTT assay, and absorbance of the resulting formazan product was measured at 450 nm with a plate reader. Viability values for vehicle-treated cells were calculated as percentages relative to the untreated control cells. Bars represent mean ± SD ( n = 4). Data were analysed using an unpaired t-test (GraphPad Prism). ns, not significant

Article Snippet: Data were analysed by the one-way analysis of variants (ANOVA) followed by Dunnett’s multiple comparison test, or by Student’s t-test, using the GraphPad prism software package (version 9).

Techniques: Incubation, Control, MTT Assay

Journal: BMC Complementary Medicine and Therapies

Article Title: Bioactivity-guided fractionation of Withania somnifera (L.) Dunal roots extract: evaluation of the anti-progressive potential on human Kelly neuroblastoma cell line

doi: 10.1186/s12906-025-05018-2

Figure Lengend Snippet: Percentage viability of Kelly cells treated with W. somnifera . Cells were incubated for 96 h in 96-well plates with medium containing the vehicle (vehicle control) or vehicle supplemented with various concentrations of W. somnifera extract ( A ) or fractions 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, and 12 ( B to L ). Cell viability was assessed by MTT assay, and absorbance of the resulting colour was measured at 450 nm with a plate reader. Viability values for treated cells were expressed as percentages relative to vehicle-treated controls. Bars represent mean ± SD ( n = 4). Data were analysed by one-way ANOVA followed by Dunnett’s test (GraphPad Prism). P values ≤ 0.05 were considered statistically significant; ns indicates not significant. Asterisks below the x-axes denote the determined sub-cytotoxic concentrations

Article Snippet: Data were analysed by the one-way analysis of variants (ANOVA) followed by Dunnett’s multiple comparison test, or by Student’s t-test, using the GraphPad prism software package (version 9).

Techniques: Incubation, Control, MTT Assay

Journal: BMC Complementary Medicine and Therapies

Article Title: Bioactivity-guided fractionation of Withania somnifera (L.) Dunal roots extract: evaluation of the anti-progressive potential on human Kelly neuroblastoma cell line

doi: 10.1186/s12906-025-05018-2

Figure Lengend Snippet: Linearity of liberated calcein fluorescence versus Kelly cell number. Kelly cells were seeded overnight in 96-well plates at 37 °C. Cells were subsequently incubated for one hour with Cultrex cell dissociation buffer containing calcein-AM at 37 °C. Liberated calcein fluorescence was measured using a plate reader at an excitation wavelength of 485 nm and an emission wavelength of 520 nm. Data points represent mean ± SD ( n = 4). The coefficient of determination (R²) was calculated using nonlinear regression analysis (GraphPad Prism)

Article Snippet: Data were analysed by the one-way analysis of variants (ANOVA) followed by Dunnett’s multiple comparison test, or by Student’s t-test, using the GraphPad prism software package (version 9).

Techniques: Fluorescence, Incubation

Journal: BMC Complementary Medicine and Therapies

Article Title: Bioactivity-guided fractionation of Withania somnifera (L.) Dunal roots extract: evaluation of the anti-progressive potential on human Kelly neuroblastoma cell line

doi: 10.1186/s12906-025-05018-2

Figure Lengend Snippet: Linearity of Kelly cell adhesion to fibronectin. Adhesion assays were performed in 24-well plates pre-coated with fibronectin. After blocking non-specific binding sites, Kelly cells were seeded at densities ranging from 0.625 to 10 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\:\times\:$$\end{document} 10⁵ cells/mL and incubated for 40 min at 37 °C. Adherent cells were stained with crystal violet dye, followed by lysis, and absorbance of the lysates was measured at 595 nm using a plate reader. Data points represent mean ± SD ( n = 4). The coefficient of determination (R²) was calculated using nonlinear regression analysis (GraphPad Prism)

Article Snippet: Data were analysed by the one-way analysis of variants (ANOVA) followed by Dunnett’s multiple comparison test, or by Student’s t-test, using the GraphPad prism software package (version 9).

Techniques: Blocking Assay, Binding Assay, Incubation, Staining, Lysis

Journal: BMC Complementary Medicine and Therapies

Article Title: Bioactivity-guided fractionation of Withania somnifera (L.) Dunal roots extract: evaluation of the anti-progressive potential on human Kelly neuroblastoma cell line

doi: 10.1186/s12906-025-05018-2

Figure Lengend Snippet: Viability of Kelly Cells treated with W. somnifera subfractions 9 and 10. Kelly cells were incubated for 96 h in 96-well plates with either medium containing 0.5% DMSO (vehicle) or the vehicle supplemented with various concentrations of W. somnifera subfractions: 9/1 ( A ), 9/2 ( B ), 9/3 ( C ), 10/1 ( D ), 10/2 ( E ), and 10/3 ( F ). Following incubation, cell viability was assessed using the MTT assay. Absorbance of the resulting colour was measured at 450 nm using a microplate reader. Viability percentages were calculated relative to vehicle-treated cells. Data are presented as mean ± SD ( n = 4). Statistical analysis was performed using ANOVA followed by Dunnett’s test (GraphPad Prism). P ≤ 0.05 were considered statistically significant; “ns” indicates non-significance. Asterisk below the x-axes indicates sub-cytotoxic concentrations

Article Snippet: Data were analysed by the one-way analysis of variants (ANOVA) followed by Dunnett’s multiple comparison test, or by Student’s t-test, using the GraphPad prism software package (version 9).

Techniques: Incubation, MTT Assay